Bimax on yeast
Bimax is an exhaustive biclustering method, finding hundreds of up-regulation constant biclusters on the yeast data. The overlapper reveals two 'superbiclusters', or groups of genes and conditions, that Bimax recurrently groups together
Three biclusters overlapped. There is a common area for the three and areas shared by 1, 2 and 2, 3. The tool capability to highlight this kind of superstructures (we will call them 'superbiclusters') in biclustering results is one of their most intereseting features.
Representation of the 100 biggest biclusters (left) and 50 biggest biclusters (right) of Bimax for Eisen yeast microarray data. Two groups appear at top (1) and bottom (2) of the figures, with only some genes active in both. The addition of biclusters make groups tighter, reflecting the exhaustiveness of Bimax (much of the biclusters found are very similar).
Same example, but up to 250 biclusters!. The groupings are still clear, even clearer without hulls. Some genes and conditions reveals as really important for Bimax (piecharts with lots of radial lines).
If only conditions are represented, the two groups are unconnected, and visualization is simplified. Now it's even clearer than sporulation conditions and heat conditions are the ones that cause over-expression, since this is the criterium for Bimax biclustering. It's interesting how heat.0 is related to sporulation conditions and not to the heat conditions. |